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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1449 |
---|---|
产品名称 | Human PD-L1 ELISA Kit |
产品描述 | For quantitative detection of human PD-L1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, F19-R238 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <12pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CD274 |
基因全名 | CD274 molecule |
Uniprot ID | Q9NZQ7 |
指标别名 | B7 H|B7H|B7 H1|B7-H1|B7H1|CD 274|CD274|PD L1|PD-L1|PDL 1|PDL1 |
背景资料 | Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1449-CAP | 96-well plate precoated with anti- human PD-L1 antibody | 1 |
EK1449-ST | Lyophilized recombinant human PD-L1 standard | 10ng/tube×2 |
EK1449-DA | Biotinylated anti- human PD-L1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 168 | 556 | 1564 | 151 | 519 | 1408 |
Standard deviation | 8.23 | 23.35 | 79.76 | 10.26 | 22.31 | 97.15 |
CV(%) | 4.9% | 4.2% | 5.1% | 6.8% | 4.3% | 6.9% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.003 | 0.055 | 0.101 | 0.183 | 0.388 | 0.744 | 1.272 | 2.194 |
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