手机验证
发货周期:7-8周
博士德生物
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Human
96T
货号 | EK2160 |
---|---|
产品名称 | Human CDSN ELISA Kit |
产品描述 | For quantitative detection of human CDSN in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
免疫原 | Expression system for standard: NS0; Immunogen sequence: K33-P529 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 31.2 pg/ml - 2,000 pg/ml |
基因名称 | CDSN |
基因全名 | corneodesmosin |
Uniprot ID | CDSN: Q15517 |
指标别名 | CDSN; Corneodesmosin; S protein |
背景资料 | Corneodesmosin is a protein that in humans is encoded by the CDSN gene. This gene encodes a protein found in corneodesmosomes, which localize to human epidermis and other cornified squamous epithelia. The encoded protein undergoes a series of cleavages during corneocyte maturation. This gene is highly polymorphic in human populations, and variation has been associated with skin diseases such as psoriasis, hypotrichosis and peeling skin syndrome. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2160-CAP | 96-well plate precoated with anti- Human CDSN antibody | 1 |
EK2160-ST | Lyophilized recombinant Human CDSN standard | 10ng/tube×2 |
EK2160-DA | Biotinylated anti- Human CDSN antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | ||||||
Sample 1 | 42 | 62 | 52 | 48 | 51 | 1.84 | 3.6% |
Sample 2 | 155 | 167 | 185 | 172 | 170 | 9.347 | 5.5% |
Sample 3 | 697 | 702 | 754 | 784 | 734 | 35.98 | 4.9% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
O.D. | 0.016 | 0.033 | 0.065 | 0.122 | 0.289 | 0.594 | 1.052 | 1.887 |
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