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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1509 |
---|---|
产品名称 | Human Persephin ELISA Kit |
产品描述 | For quantitative detection of human Persephin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, W22-G156 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 93.7pg/ml-6000pg/ml |
基因名称 | PSPN |
基因全名 | persephin |
Uniprot ID | O60542 |
指标别名 | Persephin | PSP | PSPN | O60542 |
背景资料 | Persephin, also known as PSPN, is a neurotrophic factor in the glial cell line-derived neurotrophic factor (GDNF) family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. This PSPN gene is mapped to chromosome 19p13.3. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1509-CAP | 96-well plate precoated with anti- human Persephin antibody | 1 |
EK1509-ST | Lyophilized recombinant human Persephin standard | 10ng/tube×2 |
EK1509-DA | Biotinylated anti- human Persephin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 175 | 597 | 3887 | 176 | 600 | 4002 |
Standard deviation | 8.4 | 35.82 | 295.41 | 8.62 | 37.8 | 352.17 |
CV(%) | 4.8% | 6% | 7.6% | 4.9% | 6.3% | 8.8% |
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.017 | 0.122 | 0.185 | 0.351 | 0.725 | 1.004 | 1.780 | 2.015 |
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