The antibody was purified from antisera by immunoaffinity chromatography.
Conjugation
Alkaline Phosphatase
Form
Concentrated, Liquid
Tested Application
WB,ELISA
Contents
1mg/ml of Alkaline Phosphatase conjugated specific antibody;0.05M Tris buffer, pH8.0, containing 1% BSA, 1mM MgCl2, 50% glycerol, with 15mM sodium azide as a preservative.
Suggested Working Concentration
Western Blotting: 1:3000-10000 (ECL)Western Blotting: 1:300-1500(DAB detection)Direct ELISA: 1:5000-30000(TMB detection)Optimal working dilutions must be determined by end users.