1mg/ml of HRP conjugated specific antibody;0.01M PBS, 50% glycerol.
Suggested Working Concentration
Western Blotting: 1:3000-10000 (ECL)Western Blotting: 1:300-1500(DAB detection)Direct ELISA: 1:2000-10000(TMB detection)Optimal working dilutions must be determined by end users.
Application Notes
Diluted by neutral PBS or TBS.Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.