HADH2 Antibody抗体,orb214032,biorbyt

HADH2 Antibody抗体,orb214032,bio

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  • ¥1560
  • biorbyt已认证
  • orb214032
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Canine, Human, Mouse, Porcine, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      HADH2 Antibody抗体

    • 抗体英文名

      HADH2 Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Canine, Human, Mouse, Porcine, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb214032

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HADH2. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The HADH2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HADH2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:ERAB; HADH2; MRPP2; SCHAD; XH98G2; 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HADH2. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:3028, 63864

    UniProt ID:Q99714, O70351, O08756

    Note:For research use only.

    HADH2 Antibody抗体,orb214032,bio
    Western blot analysis of HADH2 expression in H446 (A), U2OS (B), DLD (C), mouse lung (D), mouse liver (E) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 27 kD)

    HADH2 Antibody抗体,orb214032,bio
    Immunohistochemical analysis of HADH2 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    HADH2 Antibody抗体,orb214032,bio
    Immunofluorescent analysis of HADH2 staining in SKNSH cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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