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Caspase 8 (Phospho-Y380) Antib

ody抗体,orb213660,biorbyt
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  • ¥1820
  • biorbyt已认证
  • orb213660
  • 英国
  • 2025年12月04日
  • IF, WB
  • Rabbit
  • Human, Monkey, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Caspase 8 (Phospho-Y380) Antibody抗体

    • 抗体英文名

      Caspase 8 (Phospho-Y380) Antibody

    • 应用范围

      IF, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Monkey, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb213660

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y380 of human Caspase 8 protein. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The Caspase 8 (Phospho-Y380) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y380 of human Caspase 8 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH

    免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y380 of human Caspase 8 protein. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:841

    UniProt ID:Q14790

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Caspase 8 (Phospho-Y380) expression in HepG2 (A), Jurkat (B), mouse spleen (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 57; 18 kD)

    产品细节图片2
    Immunofluorescent analysis of Caspase 8 (Phospho-Y380) staining in B12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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