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APC Antibody抗体,orb213564,biorb

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  • ¥1560
  • biorbyt已认证
  • orb213564
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Gallus, Human, Porcine, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      APC Antibody抗体

    • 抗体英文名

      APC Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Gallus, Human, Porcine, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb213564

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human APC. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The APC Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human APC. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human APC. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:24205, 324

    UniProt ID:P25054, P70478

    Note:For research use only.

    产品细节图片1
    Western blot analysis of APC expression in U87MG (A), HCT116 (B) whole cell lysates. (Predicted band size: 311 kD; Observed band size: 310 kD)

    产品细节图片2
    Immunohistochemical analysis of APC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of APC staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

      酶活性的DNA聚合酶,原来通常使用的是Taq DNA聚合酶,缺少校正功能。在这儿我们展示将长PCR技术应用到5'RACE系统中,来分离5.5kb长的人结节性脑硬化II(tuberous sclerosis II,TSC-2)mRNA和8.9kb长的腺瘤性结肠息肉(adenomatous polyposis coli,APC)mRNA的5'末端,扩增的5'末端片段要比单独用Taq DNA聚合酶获得的片段长。方法使用5'RACE系统版本2 (Cat. No. 18374-058)来扩增TSC-2和APC

    • APC

      APC 由阿斯匹林( Aspirin )、非那西汀( Phenacetin )与咖啡因( Caffeine )组成。它有三个主要的生理功能:止痛、退热降温和消炎(如活动性风湿症、类风湿性关节炎)。阿斯匹林的化学名为乙酰水杨酸,由水杨酸(即邻羟基苯甲酸)与醋酐反应而制得:   其实,水杨酸本身已具有上述生理功能,但酚基会刺激人的嘴和咽喉。在水杨酸分子中— OH 被转变为 基,便消除了水杨酸的副作用。服用后进入消化系统,它又转变成水杨

    • 非专职APC

      非专职 APC       一类各自具有特定功能,诱导后可参与提呈抗原的细胞。在通常条件下,这类细胞执行其功能而不表达MHCⅡ类分子,因而不具备提呈抗原的能力。但在细胞因子和病原体的作用下,特别在炎症反应持续的阶段中,可被诱导而表达为抗原提呈所必需的MHCⅡ类分子和共信号分子,以非专职形式行使抗原提呈功能。属于非专职APC的,有血管内皮细胞、皮肤成纤维细胞、皮肤角质形成细胞、胸腺上皮细胞、甲状腺上皮细胞、胰岛β细胞、脑小胶质细胞等。       血管

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