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CDC25C Antibody抗体，orb216053，bi

orbyt
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  • ￥1560
  • biorbyt已认证
  • orb216053
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 技术资料
    • 抗体名：

      CDC25C Antibody抗体

    • 抗体英文名：

      CDC25C Antibody

    • 应用范围：

      IF, IHC, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb216053

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原：

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary.

    • 规格：

      30 ul

    产品描述：The CDC25C Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C

    免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary.

    应用稀释比例：WB: 1:500-1:1000, IHC-P: 1:100-1-200, IF/ICC: 1:100-1:500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:12532, 995

    UniProt ID:P30307, P48967

    Note:For research use only.

    产品细节图片1
    Western blot analysis of CDC25C expression in rat brain (A), mouse lung (B), CT26 (C), PC12 (D), U87MG (E), Hela (F) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 75 kD)

    产品细节图片2
    Immunohistochemical analysis of CDC25C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of CDC25C staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    • CHK1 Kinase Activity Assay

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    • A Xenopus Cell-Free System for Analysis of the Chfr Ubiquitin Ligase Involved in Control of Mitotic Entry

      1) for degradation, leading to delayed activation of the Cdc25C phosphatase and prolonged inhibitory phosphorylation of Cdc2 at the G2/M transition. In this chapter, we will describe biochemical methods we developed to analyze the Chfr auto-ubiquitination activity

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