Stem Cell Transcription Factors RT² Profiler™ PCR Array profiles the expression of 84 key genes associated with stem cell differentiation and development. During development, a few key transcription factors (NANOG, POU5F1, and SOX2) drive embryonic stem cell maintenance. These genes activate or repress other key transcription factors represented by this array that are necessary for development and differentiation, forming a transcriptional regulatory network. For example, embryonic stem cell maintenance requires POU5F1 (OCT4), whereas trophectoderm development requires CDX2. These two genes repress each other, forming a regulatory network that either delays development or allows it to proceed as signaled. Understanding these regulatory loops is essential to decipher developmental transcriptional networks and ultimately elucidate differentiation mechanisms. This array includes key transcription factors necessary for stem cell maintenance, as well as transcription factors and cofactors involved in and regulated during developmental processes. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of transcription factors involved in stem cell differentiation and development with this array.