干细胞转录因子PCR基因芯片 Stem Cell Trans

“英拜为您实验加速”
技术服务网址：http://www.yingbio.com/
服务热线：400-696-6643、 18019265738
邮箱：daihp@yingbio.com 、 huizhang1228@foxmail.com

Stem Cell Transcription Factors PCR Array

干细胞转录因子PCR基因芯片

Product	Species	Technology	Cat. No.
Stem Cell Transcription Factors PCR Array	Human	Gene Expression	PAHS-501Z
Stem Cell Transcription Factors PCR Array	Mouse	Gene Expression	PAMM-501Z
Stem Cell Transcription Factors PCR Array	Rat	Gene Expression	PARN-501Z

Stem Cell Transcription Factors RT² Profiler™ PCR Array profiles the expression of 84 key genes associated with stem cell differentiation and development. During development, a few key transcription factors (NANOG, POU5F1, and SOX2) drive embryonic stem cell maintenance. These genes activate or repress other key transcription factors represented by this array that are necessary for development and differentiation, forming a transcriptional regulatory network. For example, embryonic stem cell maintenance requires POU5F1 (OCT4), whereas trophectoderm development requires CDX2. These two genes repress each other, forming a regulatory network that either delays development or allows it to proceed as signaled. Understanding these regulatory loops is essential to decipher developmental transcriptional networks and ultimately elucidate differentiation mechanisms. This array includes key transcription factors necessary for stem cell maintenance, as well as transcription factors and cofactors involved in and regulated during developmental processes. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of transcription factors involved in stem cell differentiation and development with this array.

干细胞转录因子PCR基因芯片可以同时检测与干细胞分化和发育相关的84个重点基因的表达。在发育过程中，有几个关键的转录因子（NANOG，POU5F1，SOX2）维持着胚胎干细胞。该芯片检测的这些基因可激活或抑制相应的发育和分化所必需的转录因子，并形成一个复杂的转录调控网络。例如，胚胎干细胞的维护需要POU5F1（OCT4），而滋养外胚层发展需要CDX2。这两个基因互相压制对方，并形成一个调控网络，共同调节细胞发育延迟或允许它进行分化。了解这些调控环路，对于最终破译发育的基因调控网络、阐明细胞分化机制至关重要。该芯片包括维护干细胞，以及在发育过程中所必需的转录因子和辅助因子。利用实时定量PCR，研究者可以方便并且可信地对与干细胞分化和发育相关的转录因子进行同时检测。

Somatic Stem Cell Maintenance:CDX2, NANOG, POU5F1 (OCT4), SOX2.
Placenta Development:CDX2, HAND1, PPARG, SP1, VDR.
Induced Pluripotent & Embryonic Stem Cells:NANOG, POU5F1, SOX2, STAT3.
Axis / Symmetry / Segmentation:CDX2, DLX1, DLX2, FOXA2, HOXA11, HOXA2, HOXA3, HOXA7, HOXA9,HOXA10, HOXB1, HOXB3, HOXB5, HOXB8, HOXC10, HOXC4, HOXC5, HOXC6, HOXC9, HOXD10, HOXD4,LMX1B, NEUROD1, NOTCH2, NR2F2, PAX1, PITX2, SMAD2, TBX5, TDGF1, ZIC1.
Embryonic Development:CDX2, DLX1, DLX2, FOXA2, GATA6, GLI2, HAND1, HOXA11, HOXA2, HOXA3,HOXA7, HOXA9, HOXA10, HOXB1, HOXB3, HOXB5, HOXB8, HOXC10, HOXC4, HOXC5, HOXC6, HOXC9,HOXD1, HOXD10, HOXD4, KLF4, LMX1B, MSX2, NANOG, NEUROD1, NOTCH2, PAX1, SIX2, SMAD2, SOX2,SP1, TBX5, TDGF1, ZFPM2, ZIC1.
Ectoderm, Endoderm & Mesoderm Formation & Differentiation:FOXA2, GATA6, HAND1, HOXA7, HOXB13,ISL1, KLF4, SMAD2, SOX9.
Organ Morphogenesis:CDX2, DLX1, DLX2, GLI2, HAND1, HOXA11, HOXA2, HOXA3, HOXA7, HOXB1,HOXB13, HOXB3, HOXB5, HOXB8, HOXC4, HOXC9, HOXD10, HOXD4, ISL1, JUN, KLF4, MSX2, MYC,NEUROD1, NOTCH2, NR2F2, PAX1, PAX5, PAX6, PITX2, PITX3, PPARG, RUNX1 (AML1), SIX2, SMAD2, SOX2,SOX9, SP1, STAT3, TBX5, TDGF1, VDR, WT1, ZFPM2, ZIC1.
Angiogenesis:CDX2, HAND1, JUN, NR2F2, RUNX1 (AML1), WT1.
Neurogenesis:DLX1, DLX2, DNMT3B, FOXA1, FOXA2, FOXP3, GLI2, HOXA2, HOXC10, HOXD10, ISL1, LMX1B,NEUROD1, NKX2-2, NR2F2, OLIG2, PAX6, PITX3, POU4F1, POU4F2, PPARG, SOX2, STAT3, TLX3.
Hematopoiesis:RB1, RUNX1 (AML1), SOX6, SP1, STAT1.
Osteogenesis:GLI2, HOXA2, SOX2, SP1.
Other: DACH1, EGR3, ESR1, EZH2, FOXP1, FOXP2, GATA1, HOXC12, HTR7, IRX4, KLF2, LIN28B, NFATC1,PAX9, PCNA, TERT, WRN.