氧化应激PCR基因芯片 Oxidative Stress P

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Oxidative Stress PCR Array

氧化应激PCR基因芯片

Product	Species	Technology	Cat. No.
Oxidative Stress Plus PCR Array	Human	Gene Expression	PAHS-065Y
Oxidative Stress PCR Array	Human	Gene Expression	PAHS-065Z
Oxidative Stress PCR Array	Mouse	Gene Expression	PAMM-065Z
Oxidative Stress PCR Array	Rat	Gene Expression	PARN-065Z
Oxidative Stress PCR Array	Dog	Gene Expression	PAFD-065Z
Oxidative Stress PCR Array	Zebrafish	Gene Expression	PAZF-065Z
Oxidative Stress PCR Array	Chinese Hamster	Gene Expression	PAJJ-065Z

Oxidative Stress Plus RT² Profiler PCR Array profiles the expression of 84 genes related to oxidative stress. It also determines whether oxidative stress pathway activity is increased or unchanged in experimental samples. Peroxidases are represented on this array, including glutathione peroxidases (GPx) and peroxiredoxins (TPx). Also included are the genes involved in reactive oxygen species (ROS) metabolism, such as oxidative stress responsive genes and genes involved in superoxide metabolism such as superoxide dismutases (SOD). The array also includes 16 experimentally derived Signature Biomarker Genes which, along with classification algorithms, are used to generate the activity score. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably determine oxidative stress pathway activity and analyze expression of a focused panel of genes related to oxidative stress with this array.
氧化应激增强版PCR芯片可以用于检测84个与氧化应激相关的基因，以及确定实验样品中的氧化应激信号通路活性是否增加或者发生变化。该芯片涵盖的基因有：过氧化物酶，比如谷胱甘肽过氧化物酶（GPx）和peroxiredoxins（TPX）；与氧自由基（ROS）代谢相关的基因，比如氧化应激反应基因；与超氧化物代谢相关的基因，比如超氧化物歧化酶（SOD）；此外，这款芯片还包含了16个经过实验验证的分子标记物基因，通过这16个基因的表达数据以及特定的分类算法，可以计算样品中该信号通路的活性分值。每张芯片中还包含了一系列内参，以便于客户通过ΔΔCT法对数据进行相对定量分析，评估逆转录和PCR的效率，判断是否存在基因组DNA污染。通过实时定量PCR方法，研究者可以方便并且可信地判断氧化应激通路的活性，并对相关基因的表达进行集中的分析。
Antioxidants:
Glutathione Peroxidases (GPx): GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, GPX7, GSTP1, GSTZ1.
Peroxiredoxins (TPx): PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 (AOP2).
Other Peroxidases: CAT, CYBB, CYGB, DUOX1, DUOX2, EPX, LPO, MGST3, MPO, PTGS1, PTGS2 (COX2), PXDN, TPO, TTN.
Other Antioxidants: ALB, APOE, GSR, MT3, SELS, SOD1, SOD3, SRXN1, TXNRD1, TXNRD2.
Genes Involved in Reactive Oxygen Species (ROS) Metabolism:
Superoxide Dismutases (SOD): SOD1, SOD2, SOD3.
Other Genes Involved in Superoxide Metabolism: ALOX12, CCS, DUOX1, DUOX2, GTF2I, MT3, NCF1, NCF2, NOS2 (iNOS), NOX4, NOX5, PREX1, UCP2.
Other Genes Involved in ROS Metabolism: AOX1, BNIP3, EPHX2, MPV17, SFTPD.
Oxidative Stress Responsive Genes: APOE, ATOX1, CAT, CCL5 (RANTES), CYGB, DHCR24, DUOX1, DUOX2, DUSP1 (PTPN16), EPX, FOXM1, FTH1, GCLC, GCLM, GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, GPX7, GSR, GSS, HMOX1, HSPA1A, KRT1, LPO, MBL2, MPO, MSRA, NQO1, NUDT1, OXR1, OXSR1, PDLIM1, PNKP, PRDX2, PRDX5, PRDX6 (AOP2), PRNP, RNF7, SCARA3, SELS, SEPP1, SIRT2, SOD1, SOD2, SQSTM1, SRXN1, STK25, TPO, TTN, TXN, TXNRD1, TXNRD2.
Oxygen Transporters: CYGB, MB.
Pathway Activity Signature Genes: AKR1C2, BAG2, FHL2, GCLM, GLA, HMOX1, HSP90AA1, LHPP, NCOA7, NQO1, PTGR1, SLC7A11, SPINK1, TRAPPC6A, TXN, TXNRD1.