卵巢癌qBiomarke体细胞突变PCR芯片 Ovarian

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Ovarian Cancer qBiomarker Mutation PCR Array

卵巢癌qBiomarke体细胞突变PCR芯片

Product	Species	Technology	Cat. No.
Ovarian Cancer qBiomarker Mutation PCR Array	Human	Somatic Mutation	SMH-039A

The Human Ovarian Cancer qBiomarker Somatic Mutation PCR Array is a translational research tool that allows rapid, accurate, and comprehensive profiling of the somatic mutations in human ovarian cancer samples in the following key genes: BRAF, CTNNB1/beta-catenin, ERBB2, FOXL2, GNAS, KIT, KRAS, NRAS, PIK3CA, PTEN, and P53. These mutations warrant extensive investigation to enhance the understanding of carcinogenesis and identify potential drug targets. The utility of somatic mutation status information in identifying key signaling transduction disruptions has been demonstrated in numerous research studies. For example, the mutation status of the EGFR and KRAS genes can predict the physiological response to certain drugs targeting these molecules. The Human Ovarian Cancer qBiomarker Somatic Mutation PCR Array, with its comprehensive content coverage, is designed for studying mutations in the context of ovarian cancer and has the potential for discovery and verification of drug target biomarkers for this cancer type and other cancer types in which these mutations have been identified. This array includes 83 DNA sequence mutation assays designed to detect the most frequent, functionally verified, and biologically significant mutations in human ovarian cancer. These mutations were chosen from curated, comprehensive somatic mutation databases and peer-reviewed scientific literature, and represent the most frequently recurring somatic mutations compiled from over 2600 ovarian cancer samples. The simplicity of the product format and operating procedure allows routine somatic mutation profiling in any research laboratory with access to a real-time PCR instrument.
卵巢癌qBiomarke体细胞突变PCR芯片是一个翻译研究工具,用于快速,准确,全面剖析卵巢癌样本中体细胞突变的关键基因: BRAF, CTNNB1/beta-catenin, ERBB2, FOXL2, GNAS, KIT, KRAS, NRAS, PIK3CA, PTEN, and P53.这些突变保证广泛的研究,以提高致癌作用的理解和鉴定潜在的药物靶点。已有许多研究通过单个和多个体细胞突变状态信息鉴定关键信号转导中断。例如,EGFR和KRAS基因的突变状态可以预测某些药物针对这些分子的生理反应。卵巢癌qBiomarker体细胞突变PCR芯片以其全面的内容覆盖范围，用于研究卵巢癌的环境突变且有潜力用于发现靶向药物的生物标记和验证这些癌症和其他这些突变已确定的癌症。这个芯片包含83个DNA突变序列用于检测最频繁的，功能性验证，在人类卵巢癌上用有生物学意义的显著突变。这些突变的选择根据全面的体细胞突变数据库和文献，来自2600多个卵巢癌样本发生最频繁重复编译的体细胞突变。简单的产品模式和操作程序让任何一个具备实时定量PCR仪的实验室都可进行常规的体细胞突变分析。
BRAF: 1 Assay
The most important BRAF mutation in ovarian cancer leads to increased kinase activity, the p. V600E mutation.
CTNNB1: 9 Assays
The most frequently detected CTNNB1/beta-catenin mutations result in abnormal signaling in the WNT signaling pathway. The mutated codons are mainly several serine/threonine residues targeted for phosphorylation by GSK-3beta.
ERBB2: 2 Assays
The most frequently identified ERBB2 activating mutations cluster in the ERBB2 kinase domain region.
FOXL2: 1 Assay
This mutation lies in wing 2 of the forkhead domain, a divergent component of the domain's secondary structure with unknown function, but the mutation does seem to inhibit the protein's pro-apoptotic function.
GNAS: 1 Assay
Mutations in this gene result in pseudohypoparathyroidism type 1a (PHP1a), which has an atypical autosomal dominant inheritance pattern requiring maternal transmission for full penetrance.
KIT: 3 Assays
The most frequently identified KIT gain-of-function mutations include the D816V point mutation, the exon 11 (juxtamembrane domain) deletion and point mutations, an exon 9 insertion mutation, and exon 13 point mutations.
KRAS: 10 Assays
The mutation assays include the most frequently occurring mutations in KRAS codons 12, 13, and 61. Mutations at these positions result in reduced intrinsic GTPase activity and/or cause KRAS to become unresponsive to RasGAP.
NRAS: 1 Assay
The most important NRAS mutation in ovarian cancer occurs at codon 12.
PIK3CA: 7 Assays
The most frequently occurring PIK3CA mutations mainly belong to two classes: gain-of-function kinase domain activating mutations and helical domain mutations that mimic activation by growth factors.
PTEN: 3 Assays
The most commonly detected PTEN loss-of-function mutations are due to either truncation (p.R233* and p.R130*) or point mutations causing phosphatase inactivation (p.R130 and p.R173 mutations).
TP53: 45 Assays
The most frequently detected somatic mutations in TP53 are largely composed of DNA-binding domain mutations which disrupt either DNA binding or protein structure.

Overview of the qBiomarker Somatic Mutation PCR Array / Assay Protocol

Overview of the qBiomarker Somatic Mutation PCR Array / Assay Protocol.
The procedure involves DNA extraction (QIAGEN QIAamp DNA Mini Kit or FFPE Tissue Kit is recommended), an optional amplification (QIAGEN REPLI-g kit or REPLI-g UltraFast kit is recommended) step for DNA isolated from fresh samples, qPCR detection on qBiomarker Somatic Mutation PCR Arrays or Assays, and data analysis (using the qBiomarker Somatic Mutation Data Analysis Template). An optional DNA sample QC step immediately before the detection array or assay setup allows the user to qualify the DNA samples.

Principle of Mutant Discrimination with ARMS®