- 询价
- Cell Signaling Technology
- 7074
- USA
- 2025年09月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Anti-rabbit IgG, HRP-linked Antibody
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
1 ml/100 ul/5 ml/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 1 ml | 产品价格： | ￥请询价 |
| 规格： | 100 ul | 产品价格： | ￥请询价 |
| 规格： | 5 ml | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Secondary Antibodies
Anti-rabbit IgG, HRP-linked Antibody #7074
Western Blotting
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Description
Affinity purified goat anti-rabbit IgG (H&L) antibody is conjugated to horseradish peroxidase.
Applications
This product has been optimized for use as a secondary antibody in chemiluminescent western blotting applications.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Application References
- Qattan, A.T. et al. (2010) J Proteome Res 9, 495-508. Applications: Western Blotting
- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
- Dixon, A.S. et al. (2011) J Biol Chem 286, 27751-60. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7077 Anti-rat IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
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- 作者
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
人抗风疹病毒IgG抗体（anti-RV IgG）酶联免疫分析（ELISA）
中依次加入抗风疹病毒 IgG 抗体（ anti-RV IgG ），再与 HRP 标记的抗原结合，形成抗原 - 抗体 - 酶标抗原复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体（ anti-RV IgG ）呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中人抗风疹病毒 IgG 抗体（ anti-RV IgG ）浓度。 试剂盒组成
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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