Phospho-SAPK/JNK (Thr183/Tyr18

Product Pathways - MAPK Signaling

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Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IF-IC F	H M R Hm Sc	Endogenous	46, 54	Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9255:

Flow , Immunofluorescence , Immunoprecipitation , Western Blotting

Specificity / Sensitivity

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. This antibody does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK.

Background

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
2. Ichijo, H. (1999) Oncogene 18, 6087-93.
3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

Application References

• Akagi, T. et al. (2000) v-Crk activates the phosphoinositide 3-kinase/AKT pathway in transformation. Proc.Natl. Acad.Sci. USA 97, 7290-7295. Applications: Western Blotting
• Song, J. S. et al. (1999) Tyrosine phosphorylation of Vav stimulates IL-6 production in mast cells by a Rac/c-Jun N-terminal kinase-dependent C208pathway. Journal of Immunology 163, 802-810. Applications: Western Blotting
• Zhang, S. and Kaplan, M.H. (2000) The p38 mitogen-activated protein kinaseis required for IL-12-induced IFN-gamma expression. Journal of Immunology 165, 1374-1380. Applications: Western Blotting
• Xing, J. et al. (1998) Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation. Molecular and Cellular Biology 18, 1946-1955. Applications: Western Blotting
• Saporito, M. S. et al. (2000) MPTP activates c-Jun NH(2)-terminal kinase (JNK) and its upstream regulatory kinase MKK4 in nigrostriatal neurons in vivo. J. Neurochem. 75, 1200-1208. Applications: Western Blotting
• Turner, N.A. et al. (2009) Am J Physiol Heart Circ Physiol 297, H1117-27. Applications: Western Blotting
• McPherson, V.A. et al. (2009) J Immunol 183, 4940-7. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

Companion Products

• 4668 Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
• 9251 Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
• 9252 SAPK/JNK Antibody
• 9253 SAPK/JNK Control Cell Extracts
• 9258 SAPK/JNK (56G8) Rabbit mAb
• 7072 Phototope^® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9250 PhosphoPlus® SAPK/JNK (Thr183/Tyr185) Antibody Kit
• 9912 Phospho-SAPK/JNK Pathway Antibody Sampler Kit

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For Research Use Only. Not For Use In Diagnostic Procedures.