- 询价
- Cell Signaling Technology
- 4857
- USA
- 2025年11月15日
- W, IHC-P, IHC-F, IF-IC
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6
- 应用范围：
W, IHC-P, IHC-F, IF-IC
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IHC-F IF-IC | H M R | Endogenous | 32 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 235 and 236. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100ng/ml, 20 min), using Phospho-S6 Ribosomal protein (Ser235/236) (91B2) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. IHC-F (frozen)
Immunohistochemical analysis of frozen U-87MG xenograft, showing cytoplasmic localization using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. IF-IC
Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
. Biochem. J. 408:297‐315. Blenis, J., Kuo, C.J., and Erikson, R.L. 1987. Identification of a ribosomal protein S6 kinase regulated by transformation and growth‐promoting stimuli
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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