- 询价
- Cell Signaling Technology
- 4265
- USA
- 2025年12月13日
- W, IF-F
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
STOP (175) Mouse mAb
- 抗原：
purified N-STOP from rat brain
- 应用范围：
W, IF-F
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-F | H M R | Endogenous | 120 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | STOP (175) Mouse mAb detects endogenous levels of total N-STOP and O-STOP proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004). Western Blotting
Western blot analysis of extracts from wild type (WT) and N-STOP knock-out mouse brain lysates, using STOP (175) Mouse mAb (upper) or Actin Antibody (lower). (Kindly provided by Dr. Robert Margolis, Sidney Kimmel Cancer Center, San Diego, California). Western Blotting
Western blot analysis of extracts from rat brain using STOP (175) Mouse mAb. IF-F
Confocal immunofluorescent analysis of rat cerebellum (left) and forebrain (right) using STOP (175) Mouse mAb (green) and MAP2 Antibody #4542 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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