- 询价
- Cell Signaling Technology
- 4079
- USA
- 2025年09月10日
- IP
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
His-Tag (27E8) Mouse mAb (Sepharose Bead Conjugate)
- 抗原：
6xHis synthetic peptide
- 应用范围：
IP
- 库存：
大量
- 适应物种：
All
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 标记物：
Sepharose Bead
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
400 ul (40 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul (40 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP | All | Transfected Only | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide. IP
Immunoprecipitation of extracts from COS cells transfected with a construct overexpressing Tyro3-His using His-Tag (27E8) Mouse mAb (Sepharose bead conjugate) (lane 1). Mouse IgG Isotype Control (Sepharose bead conjugate) #3420 (lane 2) was used as a negative control. The western blot was probed using His-Tag Antibody #2365. |
| Description | This Cell Signaling Technology (CST) antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. His-Tag (27E8) Mouse mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
with anti c-kit MAb and the lineage-negative cells from human bone marrow are incubated with anti-CD34 and anti-CD38 MAb. With a starting population of 2–4 � 10e8 unfractionated mouse bone-marrow cells, a typical isolation procedure yields approx 2 � 10e4 FR
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
expressed in the anterior primitive endoderm of the mouse gastrula" Mechanism of Development, in press - 1997. Modified from the protocol of Harland (1991) and Current Protocols in Molecular Biology. Preparation of the probe.
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