- 询价
- Cell Signaling Technology
- 3946
- USA
- 2025年09月07日
- W, F
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Myc-Tag (71D10) Rabbit mAb (Biotinylated)
- 抗原：
synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL)
- 应用范围：
W, F
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
All
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | All | Transfected Only | 65 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Myc-Tag (71D10) Rabbit mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Western Blotting
Western blot analysis of extracts from COS cells, untransfected or transfected with Myc-chorionic somatomammotropin hormone-like 1 (CSL), using Myc-Tag (71D10) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003. Flow Cytometry
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag (71D10) Rabbit mAb (Biotinylated). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with Myc tagged protein. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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