CCRF-CEM ,T淋巴细胞

CCRF-CEM ,T淋巴细胞

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  • ¥600 - 2000
  • ATCC
  • CCL-119™
  • 2025年11月08日
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      100万

    Organism Homo sapiens, human
    Tissue peripheral blood
    Cell Type T lymphoblast
    Product Format frozen
    Morphology lymphoblast
    Culture Properties suspension
    Biosafety Level 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Disease acute lymphoblastic leukemia
    Age 4 years juvenile
    Gender female
    Ethnicity Caucasian
    Applications
    This cell line is a suitable transfection host.
    Storage Conditions liquid nitrogen vapor phase
    Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
    Subculturing
    Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 to 3 X 105 and 1 to 2 X 106 viable cells/mL.
    Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 7.5% DMSO.
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
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