- ￥5000
- GeneTex
- 美国
- GTX85472
- 2025年07月15日
- WB, ICC/IF, IP, ELISA, IHC
- Chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH).
- 亚型：
IgY
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX85472
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Chicken
- 应用范围：
WB, ICC/IF, IP, ELISA, IHC
- 浓度：
100 μg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DDDDK tag
- 抗体英文名：
DDDDK tag antibody
- 抗体名：
DDDDK tag 抗体
- 规格：
50 μg
WB analysis of RDF1 transfected cell using GTX85472 DDDDK tag antibody.
Right : Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a DDDDK epitope tag at its C-terminus
Left : Cultured HEK cells with an empty plasmid vector. Loading : 25 ug of protein loaded in each lane.
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文献和实验Mori A et al., J Biosci Bioeng 2018 (PMID:29398551)
Ojima-Kato T et al., J Biosci Bioeng 2017 (PMID:28052816)
Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
Protein Antigen Expression in Escherichia coli for Antibody Production
Escherichia coli is a frequently used expression system for the generation of protein encoded by genes from diverse kingdoms and, thus, it is well suited for the production of protein antigens for antibody generation. It is a system of choice
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