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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full-length protein expressed in 293T cell transfected with human MAP2K1 expression vector
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX84167
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF
- 浓度:
0.99 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
MEK1
- 抗体英文名:
MEK1 antibody [1F5]
- 抗体名:
MEK1 抗体 [1F5]
- 规格:
100 μl
WB analysis of wild type and MEK1 knock out HeLa cell lysate(10μg per lane) using GTX84167 MEK1 antibody [1F5].
Dilution : 1:500
ICC/IF analysis of COS7 cells transiently transfected with MEK1 plasmid using GTX84167 MEK1 antibody [1F5].
WB analysis of HEK293T cells transfected with MEK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84167 MEK1 antibody [1F5].
Loading : 5 ug per lane
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文献和实验(1 ). In our laboratory, we have developed methods to immortalize specific antibody-producing cells by fusing secreting EBV-activated lymphocytes to mouse-human heteromyeloma cell lines with electrofusion, followed by cloning (2 ). This methodology has allowed
指出不同的看家基因,让您用来确保质量。Moehlenbrock表示:“在某些情况下,我们会提供分析的建议。”例如,Sigma-Aldrich Mitochondria Isolation Kit的技术指南建议检查细胞色素c氧化酶活性和柠檬酸合酶活性,以确认外膜和内膜的完整性。 Cell Signaling Technology也提供Cell Fractionation Antibody Sampler Kit,作为分级分离试剂盒的伴随产品。生产科学家Jim Cormier介绍,当您在运行
Signal Transduction Inhibitors in Cellular Function
through membrane-bound Ras to the nucleus of the cell. In this chapter, detailed protocols for analyzing the kinase activities of the key components of the MAPKs pathway—MEK1, ERK1, JNK, and p38 MAPK—are described. A brief introduction to the chemical inhibitors
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