- ￥5600
- GeneTex
- 美国
- GTX80694
- 2025年07月13日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Mouse
- Human, Mouse, Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide sequence surrounding phosphorylated Ser140
- 亚型：
IgG1, Kappa
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX80694
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A.XS139ph (phospho Ser139)
- 抗体英文名：
Histone H2A.XS139ph (phospho Ser139) antibody [3F2]
- 抗体名：
Histone H2A.XS139ph (phospho Ser139) 抗体 [3F2]
- 规格：
100 μg
WB analysis of Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
Dilution : 1 μg/ml
IHC-P analysis of postnatal mouse (PN19) lung sections using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2]. Increased staining intensity was observed in a genetic mouse model with DNA damage in airway cells.
Antigen retrieval : heat induced epitope retrieval (HIER) method with sodium citrate buffer (pH 6.0)
Fixation : 4% PFA
Dilution : 1:400
ICC/IF analysis of cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
ICC/IF analysis of gamma irradiated HeLa cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
ICC/IF analysis of A549 cells treated with vehicle (control; 0.1% DMSO in media) or with 50 μM etoposide for 1 hour using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
Red : Primary antibody
Blue : Nuclei
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文献和实验Lee JH et al., PLoS One 2014 (PMID:24933654)
Fox JT et al., Proc Natl Acad Sci U S A 2012 (PMID:22431602)
Kinjo T et al., J Virol 2010 (PMID:20219913)
Smith S et al., PLoS One 2014 (PMID:24466340)
Wang YY et al., Cancer Chemother Pharmacol 2017 (PMID:28500555)
Wang YY et al., Head Neck 2017 (PMID:28346709)
Shang HS et al., Oncol Rep 2016 (PMID:27499229)
Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and this correlated with expression of immediate early genes suggesting that this phosphorylation is associated with transcriptional activation. Although Western
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