- ￥5800
- GeneTex
- 美国
- GTX80255
- 2025年07月09日
- WB, FACS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX80255
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS
- 浓度：
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). The antibody 87G blocks interaction of HLA-G with inhibitory receptors.,We do not recommend use of this product for Mouse,Rat.
- 抗体英文名：
HLA-G antibody [87G] (PE)
- 抗体名：
HLA-G 抗体 [87G] (PE)
- 规格：
100 μg
Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).Blocking antibodies: Column 1-2: noneColumn 3: Isotype mouse IgG2a controlColumn 4: anti-human HLA-G (87G) purifiedColumn 5: anti-human HLA-G (87G) F(ab')2 fragmentColumn 6: anti-human HLA-G (MEM-G/9)
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文献和实验Yang S et al., Cytotherapy 2020 (PMID:32811747)
Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins
in our laboratory to distinguish mRNAs encoding HLA-G5 and HLA-G6, and antibody-based protocols for identification of the soluble isoforms.
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
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