- ￥6200
- GeneTex
- 美国
- GTX78335
- 2025年07月05日
- FACS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4℃. DO NOT FREEZE. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
Phycoerythrin (PE)
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX78335
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
FACS
- 浓度：
0.1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
HLA-G
- 抗体英文名：
HLA-G antibody [MEM-G/9] (PE)
- 抗体名：
HLA-G 抗体 [MEM-G/9] (PE)
- 规格：
100 μg
FACS analysis of HLA-G1 transfectants (LCL-HLA-G1) using GTX78335 HLA-G antibody [MEM-G/9] (PE).
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文献和实验Lin YC et al., iScience 2023 (PMID:36876120)
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins
in our laboratory to distinguish mRNAs encoding HLA-G5 and HLA-G6, and antibody-based protocols for identification of the soluble isoforms.
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
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