![Nuclear Matrix Protein p84 antibody [5E10]](https://img1.dxycdn.com/2022/0328/719/8464917356663500453-14.jpg)
- ￥4300
- GeneTex
- 美国
- GTX70220
- 2025年07月14日
- WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
- Mouse
- Human, Mouse, Rat, Hamster, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Amino acids 15-374 of human p84 expressed in E. coli.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Hamster, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70220
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Knockdown/Knockout validation was supported by references (PMID:25296641)
- 抗体英文名：
Nuclear Matrix Protein p84 antibody [5E10]
- 抗体名：
Nuclear Matrix Protein p84 抗体 [5E10]
- 规格：
100 μl
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (GTX70220) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Sample (50 μg of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.
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