- ￥4300
- GeneTex
- 美国
- GTX70218
- 2025年07月08日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
- Mouse
- Human, Mouse, Cat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Gel-purified p53 containing p53 amino acids 14-389 (derived from the pSV53C p53 cDNA clone).
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Cat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70218
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
- 浓度：
2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
p53
- 抗体英文名：
p53 antibody [Pab240]
- 抗体名：
p53 抗体 [Pab240]
- 规格：
100 μl
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab240] (GTX70218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab240] (GTX70218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX70218 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53(GTX70218) antibody at 1:200 dilution.
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文献和实验Tien YT et al., Vet J 2015 (PMID:25616642)
Park D et al., Korean J Physiol Pharmacol 2022 (PMID:36039738)
Kim JW et al., J Ethnopharmacol 2021 (PMID:34265383)
Moon D et al., Anat Cell Biol 2019 (PMID:31598361)
Daeun Moon et al., ACB 2019 52()
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豚鼠总P53蛋白(Total P53)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠总P53 蛋白 ( Total P53 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 P53 单抗包被于酶标板上，标准品和样品中的 P53与单抗结合，加入生物素化的抗豚鼠 P53 ，形成免疫复合物连接在板上，辣根
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