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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human PI3 kinase p55 gamma. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX107779
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PI3 kinase p55 gamma
- 抗体英文名:
PI3 kinase p55 gamma antibody
- 抗体名:
PI3 kinase p55 gamma 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PIK3R3(GTX107779) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (30μg whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107779 diluted at 1:1000
PIK3R3 antibody detects PIK3R3 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
PIK3R3 antibody (GTX107779) dilution: 1:1000
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文献和实验Yuan L et al., J Cell Biochem 2019 (PMID:31297863)
Bao J et al., Cancer Res 2019 (PMID:30352814)
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Immunoprecipitation and Immune Complex kinase assay
---------------------------------------------------------------------------- KINASE ASSAY 1) Resuspend pellets in 10 microliters of kinase buffer (40 mM PIPES, pH 7.0; 10 mM MnCl2). Keep samples on ice until use. 2) Make up a ATP mix such that you have 2 or 10 microcuries of gamma-[32P] ATP
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
of kinase buffer (40 mM PIPES,pH 7.0; 10 mM MnCl2).Keep samples on ice until use. 2)Make up a ATP mix such that you have 2 or 10 microcuries of gamma-[32P] ATP per 10 microliters KB.When using an exogenous substrate such as enolase,add the substrate
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