- ￥4300
- GeneTex
- 美国
- GTX70115
- 2025年07月08日
- WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70115
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
- 浓度：
2.75 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
- 抗体英文名：
BRCA1 antibody [6B4] - ChIP grade
- 抗体名：
BRCA1 抗体 [6B4] - ChIP grade
- 规格：
100 μl
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.
Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70115) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
BRCA1 antibody [6B4] (GTX70115) was used at 1:1000 dilution for western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody [13B3] (GTX70228) was used as a loading control.
BRCA1 antibody 6B4 (GTX70115) was used for immunofluorescent staining of BRCA1 nuclear foci induced by ionizing radiation.
IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining.
DAPI was used to counterstain nucleus.
6B4 was used at 1:400 dilution.
Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4).
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文献和实验Li S et al., Nature 2000 (PMID:10910365)
Lajud SA et al., Clin Cancer Res 2014 (PMID:25324139)
Bernard-Gallon DJ et al., Anticancer Res 2004 (PMID:15015615)
Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
Characterization and Quality Control of Antibodies Used in ChIP Assays
, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines
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