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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX70105
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IHC-P, IP
- 浓度:
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ATM
- 抗体英文名:
ATM antibody [3E8]
- 抗体名:
ATM 抗体 [3E8]
- 规格:
100 μl
Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 μg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [3E8] (GTX70105) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.
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文献和实验Bai L et al., Gastroenterology 2006 (PMID:16952553)
Shiotani B et al., Mol Cancer Res 2006 (PMID:16513843)
Fini L et al., Carcinogenesis 2008 (PMID:17999988)
Analyzing the Regulation and Function of ATM
activity is measured using p531–44 -specific substrate or by immunoblotting extracts with an anti-phosphoserine 15 p53-specific antibody. Missense mutations affecting ATM kinase activity are detected using in vitro mutagenesis of ATM cDNA followed
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domains as well as ataxia telangiectasia mutated (ATM) protein kinase in cells treated with inhibitors of DNA replication. Phosphorylation of these proteins is detected in individual cell immunocytochemically with phospho-specific antibody (Ab
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