- ￥1700 - 4000
- GeneTex
- 美国
- GTX631830
- 2025年07月14日
- WB, ICC/IF, IHC-P, IHC-Fr, IP
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX631830
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
beta III Tubulin antibody [GT886] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.
- 抗体英文名：
beta Tubulin 3/ Tuj1 antibody [GT886]
- 抗体名：
beta Tubulin 3/ Tuj1 抗体 [GT886]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
beta Tubulin 3/ TUJ1 antibody [GT886] detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT886] (GTX631830) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% PFA at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250.
Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT886] (GTX631830).
Western blot analysis was performed using beta III Tubulin antibody [GT886] (GTX631830).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631830) diluted at a dilution of 1:1000.
beta III Tubulin antibody [GT886] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000.
Red: Sox2 antibody (GTX101506) diluted at 1:250.
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.
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文献和实验Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
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