Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
亚型:
IgG2b
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX629627
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, FACS
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
p21 Cip1
抗体英文名:
p21 Cip1 antibody [GT8611]
抗体名:
p21 Cip1 抗体 [GT8611]
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
p21 Cip1 antibody [GT8611] (GTX629627) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
p21 Cip1 antibody [GT8611] detects p21 Cip1 protein by Western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24 hr) 15 % SDS-PAGE p21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:1000
p21 Cip1 antibody [GT8611] detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells treated with 30μM cisplatin for 24hr and were fixed in 4% PFA at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. Blue: Hoechst 33342 staining.
Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.