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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length GFP recombinant protein
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
HRP
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX628528-01
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody reacts with GFP variants including GFP, EGFP, and YFP.
- 抗体英文名:
GFP antibody [GT859] (HRP)
- 抗体名:
GFP 抗体 [GT859] (HRP)
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
GFP antibody (HRP) detects GFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
GFP antibody (HRP) (GTX628528-01) dilution: 1:10000
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文献和实验Gorinski N et al., Nat Commun 2019 (PMID:31477731)
Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
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