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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Zebrafish
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX627404
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SOX2
- 抗体英文名:
SOX2 antibody [GT1876]
- 抗体名:
SOX2 抗体 [GT1876]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg zebrafish eye lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:500
SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000
SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).
Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
10% SDS PAGE
GTX627404 diluted at 1:5000
Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.
SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.
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文献和实验Li HK et al., Sci Rep 2016 (PMID:27344963)
Mashiko T et al., Cells 2021 (PMID:33806638)
Zhang L et al., Cancer Lett 2021 (PMID:33309780)
Lee KY et al., Cells 2020 (PMID:33375719)
Ma L et al., J Cell Mol Med 2020 (PMID:33108691)
Andreucci E et al., J Mol Med (Berl) 2020 (PMID:32803272)
Aminuddin A et al., Sci Rep 2020 (PMID:32398775)
Peng L et al., Int J Biol Sci 2019 (PMID:31595152)
Liangyue Peng et al., Int J Biol Sci 2019;15(11)
Lin YH et al., Endocr Relat Cancer 2018 (PMID:30400024)
Chuang HM et al., Int J Mol Sci 2018 (PMID:30287739)
Chang KY et al., Biochem Biophys Res Commun 2017 (PMID:28939040)
Huang TT et al., Oncotarget 2015 (PMID:26041881)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
Antibody Screening of Bacteriophage gt-11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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