Two KLH-conjugated synthetic peptides containing an unmodified sequence from the central part and from the C-terminus of histone H3, respectively.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Zebrafish
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60349
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ELISA, ChIP assay
靶点:
Histone H3
抗体英文名:
Histone H3 antibody - ChIP grade
抗体名:
Histone H3 抗体 - ChIP grade
规格:
50 μl
ELISA analysis of peptides used for immunization using GTX60349 Histone H3 antibody - ChIP grade.
WB analysis of whole cell extracts from HeLa cells (25 μg) using GTX60349 Histone H3 antibody - ChIP grade. Dilution : 1:500
ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60349 Histone H3 antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive MYOD1 and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).