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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX54112
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, Dot
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Histone H4K20me2 (di-methyl Lys20)
- 抗体英文名:
Histone H4K20me2 (di-methyl Lys20) antibody
- 抗体名:
Histone H4K20me2 (di-methyl Lys20) 抗体
- 规格:
100 μl
Dot blot analysis of all sorts of methylation peptides using GTX54112 Histone H4K20me2 (di-methyl Lys20) antibody.
IHC-P analysis of mouse testis tissue using GTX54112 Histone H4K20me2 (di-methyl Lys20) antibody.
Dilution : 1:200
WB analysis of various sample lysates using GTX54112 Histone H4K20me2 (di-methyl Lys20) antibody.
Loading : 25μg per lane
WB analysis of various sample lysates using GTX54112 Histone H4K20me2 (di-methyl Lys20) antibody.
Dilution : 1:1000
Loading : 25μg per lane
ICC/IF analysis of 293T cells using GTX54112 Histone H4K20me2 (di-methyl Lys20) antibody.
Blue : DAPI
IHC-P analysis of rat testis tissue using GTX54112 Histone H4K20me2 (di-methyl Lys20) antibody.
Dilution : 1:200
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文献和实验Methyl DNA Immunoprecipitation
can be done. This methylation analysis is highly specific due to the use of a well-characterized monoclonal antibody and each IP is directly quality controlled: two essential keys for reliable results. In addition, the kit protocol is fast and user-friendly. The METHYL kit
-Histone H3 和 MBD1 条带相同,简单调了下对比度;Ac-Histone H4 和 HDAC1 条带相同,180 度旋转即可。(同一个图中出现相同条带,能不能长点儿心,别把读者当傻子。。。)图片来源于论文第二篇文章于 2011 年发表,2018 年遭撤稿,目前已经被引用 118 次。图片来源于 pubmed这篇文章撤稿的主要原因同样是 WB 条带重复利用,不过这次作者聪明多了,在不同的图中出现相同条带,甚至将之前发表过的图重新利用。眼尖的小伙伴看出来了吗,3E 和 5B 的β-actin 相同
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白;在细胞正常 pH 值时,组蛋白带有正电荷,这样它们就可以和 DNA 结合,这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实,组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg , Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白: H1 , H2A , H2B , H3
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