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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Tim-3-Ig, consisting of amino acids 1-191 of mouse Tim-3, coupled with the Fc portion of mouse IgG2a.
- 亚型:
IgG3a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX54056
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
FACS
- 浓度:
0.2 mg/ml
- 靶点:
TIM-3
- 抗体英文名:
TIM-3 antibody [RMT3-23] (PE)
- 抗体名:
TIM-3 抗体 [RMT3-23] (PE)
- 规格:
100 μg
FACS analysis of C57Bl/6 splenocytes stimulated for 3 days with ConA stained APC anti-mouse CD4 antibody, followed by staining with GTX54056 TIM-3 antibody [RMT3-23] (PE).
Right panel : Co-stained with APC anti-mouse CD4 antibody and GTX54056
Left panel : Co-stained with APC anti-mouse CD4 antibody and PE rat IgG2a isotype control
Antibody amount : 0.5 μg
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文献和实验Huang S et al., Mol Ther 2021 (PMID:34933101)
Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary antibody (Typically SBA goat anti rabbit-AP 1:100, rabbit anti goat-AP 1:300) 22- wash twice 23- [optional] add a tertiary layer (rabbit anti-goat-AP on a goat anti-rabbit-AP) 24- wash thrice 25- develop AP (see protocol
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠白介素 -23(IL-23)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 IL-23 单抗包被于酶标板上,标准品和样品中的 IL-23 与单抗结合,加入生物素化的抗大鼠 IL-23 ,形成免疫复合物连接
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