- ￥5300
- GeneTex
- 美国
- GTX48643
- 2025年07月12日
- WB, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human Lefty2.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX48643
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
LEFTY2
- 抗体英文名：
LEFTY2 antibody [7C5G1H6H10]
- 抗体名：
LEFTY2 抗体 [7C5G1H6H10]
- 规格：
50 μg
Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 μg of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye™800 labeled Goat anti-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
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文献和实验Pang-Yen Liu et al., Front Pharmacol 2021 (PMID:34421617)
IMMUNOHISTOCHEMISTRY ON Drosophila BRAINS
1. Dissect brains in Drosophila Ringers solution. 2. Fix 20'' (1hr max) in a 0.5ml microfuge tube with 5 formaldehyde-PBS on ice. 3. Rinse 2-3X with PBS (carefully remove solutions with drawn out pipets). 4. Block 1h @ room
A small repository of synaptic protein information
in Drosophila Ringers solution. 2. Fix 20' (1hr max) in a 0.5ml microfuge tube with 5% formaldehyde-PBS on ice. 3. Rinse 2-3X with PBS (carefully remove solutions with drawn out pipets). 4. Block 1h @ room temperature (RT) on nutator.
A in two separate Eppies and equilibrate 3x with 500µl EBC-buffer (i.e. add EBC buffer, mix gently, spin down and remove supernatant) a. 1x for antibody binding: add 20µl RFP + 400µl EBC-buffer to equilibrated beads and incubate for 1h
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