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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD62P transfected 300.19 cells.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Goat, Sheep, Bovine, Cat, Dog, Horse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX41881
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
IHC-Fr, FACS, IP
- 浓度:
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CD62P
- 抗体英文名:
CD62P antibody [Psel.KO.2.7]
- 抗体名:
CD62P 抗体 [Psel.KO.2.7]
- 规格:
200 μg
FACS analysis of thrombin activated human platelets using GTX41881 CD62P antibody [Psel.KO.2.7].
FACS analysis of thrombin activated dog platelets using GTX41881 CD62P antibody [Psel.KO.2.7].
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文献和实验Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
tube of platelets.PROCEDURE for Flow Cytometric Analysis-Human Platelets 1. Add 100 μL of platelet suspension to the bottom of each tube.2. Add the appropriate antibody to each tube.3. Shake gently and incubate in the dark at RT for 20-30 minutes
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
in PBS. 4. Add thrombin to the cell suspension to achieve the final concentration of 0.1 to 0.2 U/ml. Incubate at room temperature for 10 minutes 5. Add equal volume of 2% formaldehyde to fix platelets for 30 minutes at room temperature. 6. Wash
In vitro mutagenesis using Altered Sites
. 8.Precipitate ssDNA with 0.5 volumes of 7.5M NH4Ac and 2 volumes EtOH. Wash pellet with 70% EtOH. 9.Resuspend the ssDNA in 20μl dH2O or TE. 10.Analyse 2μl sample by electrophoresis (M13KO7 ssDNA runs at 8.7Kb and R408 at 6.4Kb). The presence of helper phage
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