- ￥5300
- GeneTex
- 美国
- GTX27195
- 2025年07月13日
- WB, IHC-P, IHC-Fr, ELISA, IHC
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide: LRGAPSASRGSYTQQPR-C conjugated to KLH, corresponding to amino acids 1193-1209 of Mouse Gli2
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX27195
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, IHC-Fr, ELISA, IHC
- 浓度：
1.02 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Gli2
- 抗体英文名：
Gli2 antibody
- 抗体名：
Gli2 抗体
- 规格：
50 μg
GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.
GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.
Western blot using GeneTex's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 μg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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文献和实验Ma X et al., Neuroreport 2017 (PMID:27984541)
Morgan H et al., Methods Mol Biol 2018 (PMID:29675782)
Colmont CS et al., Proc Natl Acad Sci U S A 2013 (PMID:23292936)
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