- ￥4300
- GeneTex
- 美国
- GTX26655
- 2025年10月12日
- WB, ELISA, IHC
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Fluorescein conjugated to Goat IgG
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Biotin
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX26655
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Goat
- 应用范围：
WB, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Fluorescein
- 抗体英文名：
Fluorescein antibody (Biotin)
- 抗体名：
Fluorescein 抗体 (Biotin)
- 规格：
100 μg
Western Blot of Biotin Conjugated Goat Anti-Fluorescein Antibody. Lane 1: BSA conjugated Fluorescein. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.
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文献和实验Bennett M et al., J Neurotrauma 2020 (PMID:32940130)
Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
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