- ￥6600
- GeneTex
- 美国
- GTX25820
- 2025年09月08日
- WB, FACS
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide: C-LREQGRGKDQPGRLK conjugated to KLH, corresponding to amino acids 203-217 of Human Golgi marker 58K protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25820
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, FACS
- 浓度：
0.482 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognises a 58kD band in human liver lysate. The band is not seen when using the immunising peptide for blocking.
- 抗体英文名：
58K Golgi protein antibody
- 抗体名：
58K Golgi protein 抗体
- 规格：
100 μl
Flow cytometric analysis of HepG2 cells using 58K Golgi protein Antibody, pAb, Rabbit (GTX25820; shaded histogram) or with an isotype control antibody (open histogram), followed by R-PE conjugated anti-rabbit IgG.
Western blot analysis of cell and tissue lysates using 1 μg/ml Rabbit Anti-58K Golgi protein Polyclonal Antibody (GTX25820). The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 58 KD
Observed Size: 58 KD
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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
Affinity chromatography relies on the reversible interaction between a protein and a specific ligand immobilized in a chromatographic matrix. The sample is applied under conditions that favor specific binding to the ligand as the result
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