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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCbII that contains threonine 641. The sequence is conserved in mouse and rat.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX25785
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, FACS, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
PKC beta 2 (phospho Thr641)
- 抗体英文名:
PKC beta 2 (phospho Thr641) antibody
- 抗体名:
PKC beta 2 (phospho Thr641) 抗体
- 规格:
50 μl
WB (peptide competition) analysis of PMA-treated K652 cell lysates using GTX25785 PKC beta 2 (phospho Thr641) antibody with the non-phosphopeptide (Lane 2), a generic (pT) peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
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文献和实验9921 Phospho-PKC Antibody Sampler Kit 9375 Phospho-PKCalpha/beta II (Thr638/641) Antibody W IP 9374 Phospho-PKCdelta (Thr505) Antibody W IHC 9376 Phospho-PKCdelta/theta (Ser643/676) Antibody W IHC 9377 Phospho-PKCtheta (Thr538
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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