- ￥5600
- GeneTex
- 美国
- GTX25634
- 2025年07月15日
- WB, ICC/IF
- Rabbit
- Human, Mouse, Avian
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 823. The sequence is conserved in cow and dog.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Avian
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25634
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
c-Kit (phospho Tyr823)
- 抗体英文名：
c-Kit (phospho Tyr823) antibody
- 抗体名：
c-Kit (phospho Tyr823) 抗体
- 规格：
50 μl
ICC/IF analysis of Jurkat cells treated with 250 ng/ml SCF for 5 minutes using GTX25634 c-Kit (phospho Tyr823) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
WB (peptide competition) analysis of M07e cells unstimulated (Lane 1) or stimulated with 250ng/mL SCF (Lane 2-5) using GTX25634 c-Kit (phospho Tyr823) antibody with non-phosphorylated peptide (Lane 3), generic phosphotyrosine-containing peptide (Lane 4), phosphopeptide immunogen (Lane 5) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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