The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 823. The sequence is conserved in cow and dog.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Avian
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX25634
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF
浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
靶点:
c-Kit (phospho Tyr823)
抗体英文名:
c-Kit (phospho Tyr823) antibody
抗体名:
c-Kit (phospho Tyr823) 抗体
规格:
50 μl
ICC/IF analysis of Jurkat cells treated with 250 ng/ml SCF for 5 minutes using GTX25634 c-Kit (phospho Tyr823) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% PFA Permeabilization : 0.1% Triton X-100 for 10 minutes
WB (peptide competition) analysis of M07e cells unstimulated (Lane 1) or stimulated with 250ng/mL SCF (Lane 2-5) using GTX25634 c-Kit (phospho Tyr823) antibody with non-phosphorylated peptide (Lane 3), generic phosphotyrosine-containing peptide (Lane 4), phosphopeptide immunogen (Lane 5) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.