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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide YTDIEMNRLGK conjugated to KLH.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX19257
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IP, ELISA, IHC
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
VSV-G tag
- 抗体英文名:
VSV-G tag antibody
- 抗体名:
VSV-G tag 抗体
- 规格:
100 μg
WB analysis of 200, 100, and 50ng of E. coli lysate containing tagged fusion protein using GTX19257 VSV-G tag antibody.
Dilution : 1:25000
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
G coupled to agarose. In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag(Cleavage Under Targets and Tagmentation)是蛋白-DNA 互作的一大革新技术,它不需要使用甲醛交联以及免疫共沉淀,而是通过针对靶蛋白(如转录因子、染色质重塑蛋白)的抗体和 Protein A/G 的介导,使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头,经 PCR 扩增后即可形成用于高通量测序的文库(见图 1 )。CUT &Tag 技术具有细胞投入量低、信噪
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