- ￥5000
- GeneTex
- 美国
- GTX18183
- 2025年07月14日
- WB, IP, IHC
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Anti-GST antibody was produced by immunizing mice with a GST peptide.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX18183
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IP, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
GST-tag monoclonal antibody detects over expressed glutathione- transferase (GST) fusion proteins.
- 抗体英文名：
GST tag antibody [GST.B6]
- 抗体名：
GST tag 抗体 [GST.B6]
- 规格：
100 μg
Recombinant GST protein(20ng,Lane1) and 293 cellular lysates transfected with a GST expression vector(20μg,Lane2) were probed with anti-GST antibody(GTX18183)(1μg/ml).Arrow indicates GST(~26kDa) and GST-fusion protein(~29kDa).
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文献和实验一、GST pull-down 基本知识1. GST pull-down 概念GST pull-down：是利用重组技术将探针蛋白与 GST 谷胱苷肽巯基转移酶融合，融合蛋白通过 GST 与固相化在球珠上的 GSH 谷胱甘肽结合。从而分离出能与融合蛋白相互作用的蛋白的技术。2. GST pull-down 研究对象二、GST pull-down 原理1. GST pull-down 结合原理2. GST pull-down 分离原理三、GST pull-down 流程1. 蛋白的抽提与纯化：①
mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells
with 1/2 volume of 50% GST beads at 4C rocking on the Nutator for 30-60 minutes. 50 ml conical Falcon tubes work well. Pour into a BioRad Column and drain lysate. Save 100 ul of depleted lysate. Load 2.5 ul for SDS-PAGE. Wash beads with 3 column volumes
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