Recombinant protein encompassing a sequence within the center region of human PYK2. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX130887
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, IP
浓度:
1.74 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
IP/MS validation was supported by references (PMID:30377418)
抗体英文名:
PYK2 antibody
抗体名:
PYK2 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Immunoprecipitation of PYK2 protein from A431 whole cell extracts using 5 μg of PYK2 antibody (GTX130887). Western blot analysis was performed using PYK2 antibody (GTX130887) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
PYK2 antibody detects PYK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at a dilution of 1:1000.
PYK2 antibody detects PYK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2 antibody (GTX130887) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PYK2 antibody detects PYK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% PFA at RT for 15 min. Green: PYK2 protein stained by PYK2 antibody (GTX130887) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
PYK2 antibody detects PYK2 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% PFA at RT for 15 min.Green: PYK2 stained by PYK2 antibody (GTX130887) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).