- ￥1700 - 4000
- GeneTex
- 美国
- GTX130887
- 2025年07月16日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human PYK2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX130887
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1.74 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377418)
- 抗体英文名：
PYK2 antibody
- 抗体名：
PYK2 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Immunoprecipitation of PYK2 protein from A431 whole cell extracts using 5 μg of PYK2 antibody (GTX130887).
Western blot analysis was performed using PYK2 antibody (GTX130887) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
PYK2 antibody detects PYK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at a dilution of 1:1000.
PYK2 antibody detects PYK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PYK2 antibody (GTX130887) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PYK2 antibody detects PYK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: PYK2 protein stained by PYK2 antibody (GTX130887) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
PYK2 antibody detects PYK2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% PFA at RT for 15 min.
Green: PYK2 stained by PYK2 antibody (GTX130887) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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文献和实验Trenti A et al., Biochem Pharmacol 2018 (PMID:29890142)
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
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