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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha 1a Adrenergic Receptor. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Cat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100190
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
alpha 1a Adrenergic Receptor
- 抗体英文名:
alpha 1a Adrenergic Receptor antibody
- 抗体名:
alpha 1a Adrenergic Receptor 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% PFA at RT for 15 min.
Green: alpha 1a Adrenergic Receptor stained by alpha 1a Adrenergic Receptor antibody (GTX100190) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:1000
Immunofluorescence analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor(GTX100190) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded human heart, using alpha 1a Adrenergic Receptor(GTX100190) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX100190 diluted at 1:1000
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1a Adrenergic Receptor antibody (GTX100190) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Choi BR et al., Drug Des Devel Ther 2018 (PMID:29970959)
Adrenergic receptors (ARs) are G-protein-coupled transmembrane glyco-proteins that play a key role in mediating the sympathoadrenal response to stress. Pharmacological studies have suggested the existence of multiple adrenergic receptor subtypes
Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors
variety of these reagents, this becomes a relatively simple procedure. The procedure consists of a standard immunohistochemical protocol using the primary antibody against the adrenergic receptor followed by either fluorophore-tagged secondary antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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