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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human SP2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX129030
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1.65 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SP2
- 抗体英文名:
SP2 antibody
- 抗体名:
SP2 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP2 antibody (GTX129030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SP2 antibody detects SP2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
SP2 stained by SP2 antibody (GTX129030) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SP2 antibody immunoprecipitates SP2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of SP2 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SP2 by 3 μg of SP2 antibody (GTX129030) 5% SDS-PAGE The immunoprecipitated SP2 protein was detected by SP2 antibody (GTX129030) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SP2 antibody (GTX129030) diluted at 1:1000 and competitor's antibody (SC-643) diluted by 1:200.
SP2 antibody detects SP2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: SP2 stained by SP2 antibody (GTX129030) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP2 antibody (GTX129030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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