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TET2 antibody [N2], N-term

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX124204
  • 2025年07月10日
  • WB, ICC/IF, IP, ChIP assay
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX124204

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IP, ChIP assay

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      TET2

    • 抗体英文名

      TET2 antibody [N2], N-term

    • 抗体名

      TET2 抗体 [N2], N-term

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    产品细节图片2

    Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片3

    Cross-linked ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

    产品细节图片4

    TET2 antibody [N2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.
    Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
    Green: TET2 protein stained by TET2 antibody [N2], N-term (GTX124204) diluted at 1:500.
    Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
    Scale bar = 10 μm.

    产品细节图片5

    Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2], N-term (GTX124204).
    Western blot analysis was performed using TET2 antibody [N2], N-term (GTX124204) diluted at 1:500.
    EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

    产品细节图片6

    TET2 antibody detects TET2 protein by western blot analysis.
    A. 30 μg 293T whole cell lysate/extract
    B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
    5% SDS-PAGE
    TET2 antibody (GTX124204) dilution: 1:5000
    The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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